2002;292(3–4):247–56. The purpose of this article is two-fold: first, to evaluate the accuracy of a one-step H. pylori saliva antigen (HPS) test; and second, to compare noninvasive and invasive H. pylori tests in Taiwanese population. Methods We developed a fecal test to detect H. pylori based on the immunochronomatographic assay and a mixture of monoclonal antibodies. 2). PLoS ONE 2018;13(11):e0205644. Best LM, Takwoingi Y, Siddique S, Selladurai A, Gandhi A, Low B, Yaghoobi M, Gurusamy KS. government site. A next-generation sequencing-based Approach to identify genetic determinants of Antibiotic Resistance in Cambodian Helicobacter pylori Clinical isolates. CAS Then, a summary of SNPs with amino acid changes was shown, and the SNPs presented in less than 10% of the strains were excluded. The .gov means it’s official. Previous studies reported that high-biofilm formers are likely more resistant to antibiotic exposure [7, 8]. Aliment Pharmacol Ther. The 104 patients eligible for analysis (mean age: 58 years, range 22-87 years), 21 (20%) were gold standard positive. Therefore, the prediction of genetic determinants for biofilm formation is necessary. J Med Microbiol. The results also revealed several genes (Table 2) that functioned in DNA replication (DNA polymerase, DNA primase). Ansari S, Kabamba ET, Shrestha PK, Aftab H, Myint T, Tshering L, et al. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Accuracy of an enzyme immunoassay for the detection of Helicobacter pylori in stool specimens in the diagnosis of infection and posttreatment check-up. Helicobacter pylori: an up-to-date overview on the virulence and pathogenesis mechanisms. The total number of polymorphisms extracted from the analysis with the MAF > 10% was 960, including frameshift, insertion, deletion, multiple site polymorphism, and single nucleotide polymorphism, summarised in (Fig. PubMed This website, owned by Certest Biotec, S.L., uses technical cookies that allow access to the website and its services. We compiled the research findings of studies investigating the genes which play roles in biofilm formation. The purpose of this article is two-fold: first, to evaluate the accuracy of a one-step H. pylori saliva antigen … BMC Microbiology The OD of the 26695 strain biofilm was 0.35, less than 0.4, so it was also categorized as a low biofilm producer. The accessories genes associated with biofilm from whole-genome sequences were extracted and analysed, and SNPs among the previously reported biofilm-related genes were analysed. Alm RA, Bina J, Andrews BM, Doig P, Hancock RE, Trust TJ. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. -, Aliment Pharmacol Ther. Evaluation of the clinical significance of homb a novel candidate marker of Helicobacter pylori strains associated with peptic ulcer disease. Critical revision of the manuscript and final approval (all authors). Fifty-four children were H. pylori infected defined by positive culture and/or two other positive tests ((13)C-urea breath test, histology, rapid urea test), the remaining 64 children showed concordant negative results. The H. pylori stool antigen test was performed twice for each child and the results were compared to the reference method. Article Gold Standard for H. Pylori Diagnosis 2016;2(11): e000093. This study was supported in part by grants from the National Institutes of Health (DK62813) (YY) and Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan (18KK0266, 19H03473, 21H00346 and 22H02871) (Y.Y.) Would you like email updates of new search results? Stool antigen tests for the management of Helicobacter pylori infection. Characterization of monospecies biofilm formation by Helicobacter pylori. 2018 Mar 15;3(3):CD012080. 2017;3(10). Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, 879-5593, Japan, Kartika Afrida Fauzia, Ricky Indra Alfaray, Takashi Matsumoto, Michiyuki Yurugi, Evariste Tshibangu Kabamba, Junko Akada & Yoshio Yamaoka, Department of Public Health and Preventive Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia, Helicobacter pylori and Microbiota Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya, 60115, Indonesia, Kartika Afrida Fauzia, Muhammad Miftahussurur, Langgeng Agung Waskito & Ricky Indra Alfaray, Department of Gastroenterology, Dhaka Medical College and Hospital, Dhaka, 1000, Bangladesh, Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, 60115, Indonesia, Department of Physiology and Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia, Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh, 749000, Vietnam, Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, Hirosaki, 036-8562, Aomori, Japan, Research Center for Infectious Sciences, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan, Department of Medicine, Gastroenterology and Hepatology Section, Baylor College of Medicine, Houston, TX, 77030, USA, Borneo Medical and Health Research Centre, University Malaysia Sabah, Kota Kinabalu, Sabah, 88400, Malaysia, The Research Center for GLOBAL and LOCAL Infectious Diseases (RCGLID), Oita University, Yufu, 879-5593, Oita, Japan, You can also search for this author in Insertion/deletion recombinant H. pylori were first selected on chloramphenicol … Before Antos D, Crone J, Konstantopoulos N, Koletzko S. J Clin Microbiol. 17 The monoclonal antibody used in the assay reacts with … Herein, based on AC-PCR, a multiplex detection and typing system (MAC-PCR) was developed for one-step detection of H. pylori 16S rDNA and cagA gene with the purpose of H. pylori detection and cagA + genotypes identification. Upper digestive endoscopy was performed with gastric biopsy specimens for histology, a rapid urease test, culture test and quantitative real-time polymerase chain reaction. The immunoassays were independently read by two investigators. ScienceDirect® is a registered trademark of Elsevier B.V. ScienceDirect® is a registered trademark of Elsevier B.V. Journal of the Chinese Medical Association, https://doi.org/10.1016/j.jcma.2014.11.004. With increasing use of antibiotics to treat infections, mutation resistant strains have emerged in most human populations. One-step method for immune coloration, get results in 15mins. A similar tendency was observed in the strains possessing A223V and N156K substitutions in alpB genes. PubMed Central PubMed Central The variants in alpA and alpB were significantly associated with the high biofilm formation. Epub 2017 May 5. Cite this article. This study was also supported by Thailand Science Research and Innovation Fundamental Fund, Bualuang ASEAN Chair Professorship at Thammasat University, and Center of Excellence in Digestive Diseases, Thammasat University, Thailand. Article Biofilm formation and modulation of luxS and rpoD expression by Helicobacter pylori. CAS FOIA https://doi.org/10.1186/s12866-023-02889-8, DOI: https://doi.org/10.1186/s12866-023-02889-8. Please enable it to take advantage of the complete set of features! One+Step Helicobacter (H.Pylori) Antigen Test - Komplettes Test-Set zur Früherkennung Der One+Step H.Pylori-Antigen-Schnelltest (Stuhl) ist ein schneller chromatographischer Immunoassay zum qualitativen Nachweis von H.pylori-Antigenen in menschlichen Stuhlproben … 2016;11(11):e0166835. Although this has high variant, no significant hits were found in this dataset. A phylogenetic tree inferred by the maximum likelihood algorithm was used to assess the eventual association between the biofilm formation phenotype and different genetic H. pylori lineages (Fig. Oleastro M, Cordeiro R, Ferrand J, Nunes B, Lehours P, Carvalho-Oliveira I, et al. To effectively treat patients to help resolve infections, the clinician needs information on the antibiotic susceptibility profile of the infection. Cochrane Database Syst Rev. Using the minimum coverage criteria of 50% and a minimum identity of 90%, 32 of the 46 genes were present in all isolates (Supplementary Table S2). In some bacterial species, biofilm formation can be associated with specific lineages or population, e.g., in Staphylococcus aureus [27]. The reference methods showed that 23 (14.6%) of the 158 children tested were H. pylori positive. ARIBA: rapid antimicrobial resistance genotyping directly from sequencing reads. We also use third-party cookies that help us analyze and understand how you use this website. 2019 Aug 28;25(32):4629-4660. doi: 10.3748/wjg.v25.i32.4629. We evaluated the performance of a new one-step fecal test for H. pylori for diagnosis of H. pylori infection in Taiwan. 2004 Apr 15;19(8):923-9 2013;8(9):e73301. The .gov means itâs official. The x-axis represents the lists of SNPs, and the y-axis represents the p-value from fisher exact tests for the genes and the biofilm phenotype. Google Scholar. Would you like email updates of new search results? J Bacteriol. Meanwhile, finding new candidate genes among the genomes at the present-absent level is also necessary [18, 19]. Epub 2009 Oct 21. Yang DC, Blair KM, Taylor JA, Petersen TW, Sessler T, Tull CM et al. Yonezawa H, Osaki T, Kamiya S. Biofilm formation by Helicobacter pylori and its involvement for Antibiotic Resistance. Suitable for all kinds of current infection detected in people. PubMed Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. A Fisher exact test result of P-value < 0.05 was considered significant. World J Gastroenterol. Google Scholar. In addition to protecting germs from antibiotics, biofilms reduce the efficacy of H. pylori eradication therapy. Materials and methods: A gastric biopsy was collected for histopathology and rapid urease testing. Stool specimens for HpSA testing were also collected. The genes investigated in this study played a role in biofilm formation. Biofilm-forming H. pylori strains have been observed in vivo on the surface of the gastric mucosa [4]. Brynildsrud O, Bohlin J, Scheffer L, Eldholm V. Rapid scoring of genes in microbial pan-genome-wide association studies with Scoary. 2013 Dec 7;19(45):8188-91. doi: 10.3748/wjg.v19.i45.8188. The .gov means it’s official. However, it could be a stepping stone for further molecular studies elucidating the genetic factors involved in biofilm formation and related molecular mechanisms. 2011;12(3):253–8. The flagella regulation play roles in switching the planktonic and biofilm phenotype. The one-step HPS test exhibited a sensitivity of 71.43%, nearly equivalent to that of the rapid urea test. 2009;17(2):73–87. 2010 Feb 1;50(3):323-8. doi: 10.1086/649860. 2016;11(11):e0166835–e. PubMed Acquisition of data (K.A.F, H.A, M.M, M.Y, T.M, J.A, P.S), Computational method development (K.A.F, L.A.W, V.P.T, and E.T.K.). 2003 Jun;52(6):804-6 Federal government websites often end in .gov or .mil. The H. pylori genomic population has been previously described as hpAfrica1, hpAmerind, hpAsia2, hpEastAsia, and hpEurope. We use cookies to help provide and enhance our service and tailor content and ads. Real-time PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Inc.). Biofilm formation levels among the strains varied from low to high, indicating a potential complex mechanism involving a specific genotype or variant [6, 15]. Can J Gastroenterol. 2020;12(8):473. Therefore, we tried to adress this question by constructing a phylogenetic tree of Bangladeshi isolates using the SNPs-based core genome alignment tree. Epub 2016 Jun 28. One+Step Helicobacter Pylori Test Antigen Schnelltestkassette (Stuhl) - Selbstest für Zuhause, 1 Stück Preis: 14,95 € inkl. doi: 10.1177/2333794X19898345. Gastroesophageal reflux disease in an area with low Helicobacter pylori infection prevalence. MwSt. 8600 Rockville Pike Infection with Helicobacter pylori as the cause of gastric cancer is a global public health concern. CAS SNPs or missense mutations are also associated with resistance to clarithromycin, levofloxacin, and rifampicin [29]. The study was conducted following the Declaration of Helsinki and approved by the Institutional Review Board (or Ethics Committee) of Oita University Faculty of Medicine Japan with ( No: P-12-10) and the Ethics Committee of the Bangladesh Medical Research Council (BMRC) (Ref: BMRC/NREC/2013–2016/2151), Dhaka, Bangladesh. HpSA is derived from the common fragments of all sub types. Comparative Genomics revealed multiple Helicobacter pylori genes Associated with Biofilm formation in Vitro. Federal government websites often end in .gov or .mil. The Fisher exact test did not give a significant P-value. Fecal test specificity and positive predictive value were significantly higher than those of the serological test, whereas the sensitivity and negative predictive value were lower than those of the 13C urea breath test (p < 0.05). H. pylori causes more than 90% of duodenal ulcers and up to 80% of gastric ulcers. Methods: We developed a fecal test to detect H. pylori based on the immunochronomatographic assay and a mixture of monoclonal antibodies. CerTest H. pylori one step card test is a coloured chromatographic immunoassay for the qualitative detection of Helicobacter pylori in stool samples. 2007;75(8):3747–57. Article The site is secure. The Oita University Faculty of Medicine Japan and the Ethics Committee of the Bangladesh Medical Research Council (BMRC), Dhaka, Bangladesh, approved the protocol of this study. doi: 10.1002/14651858.CD012080.pub2. Google Scholar. The metadata for ARIBA was set into coding sequences and new variants. Copyright © 2023 Elsevier B.V. or its licensors or contributors. Biofilm formation was significantly associated with SNPs of alpA, alpB, cagE, cgt, csd4, csd5, futB, gluP, homD, and murF (P < 0.05). Forné M, DomÃnguez J, Fernández-Bañares F, Lite J, Esteve M, Galà N, Espinós JC, Quintana S, Viver JM. The reports were then summarised in a text file. Study of biofilm formation in C57Bl/6J mice by clinical isolates of Helicobacter pylori. A 24-well plate is loaded with 1 ml of the same bacterial solution for biofilm quantification. 2005 Jul;19(7):433-9. doi: 10.1155/2005/213608. Die One+Step Gesundheitstests sind Markenprodukte aus dem Fachhandel. Biofilm formation was quantified and classified into two groups: high- and low-biofilm formers (Supplementary Figure S1) were determined using crystal violet assay. Durchschnittliche Kundenbewertung: Helicobacter 5 Stuhltest Lks Preis: 27,55 € inkl. For example, removing the entire gene of alpB was found to inhibit the adhesion and initiation of biofilm formation [10, 11]. Yonezawa H, Osaki T, Kurata S, Fukuda M, Kawakami H, Ochiai K, et al. CerTest H. pylori card test offers a simple, a highly sensitive and non-invasive screening assay to make a presumptive diagnosis of Helicobacter pylori infection. The results of each strain consisted of the assembled genes and the report of variants compared to strain 26695. 2022 Mar;53(1):33-50. doi: 10.1007/s42770-021-00675-0. The level of genetic variation can be the present absence of genes or the SNP. Pol. Food Control. 2019; 8(1): 53. Diversification of the AlpB outer membrane protein of Helicobacter pylori affects biofilm formation and cellular adhesion. This pipeline assembles the gene according to the sequences in the references, ATCC 26695, which has low biofilm formation. This condition makes identificatation of the gene marker for the high-biofilm formers in H. pylori remains challenging. Die natürliche Behandlung von H. pylori dreht sich um eine bestimmte Ernährung, die Anwendung von Hygienemaßnahmen, die Nutzung spezifischer pflanzlicher Mittel und Probiotika sowie anderer Nahrungsergänzungsmittel. Conclusion: -. The https:// ensures that you are connecting to the J Comput biology: J Comput Mol cell biology. doi: 10.1002/14651858.CD012080.pub2. The Japan Society also supported this work for the Promotion of Science Institutional Program for Young Researcher Overseas Visits and the Strategic Funds for the Promotion of Science and Technology Agency (JST) for YY. official website and that any information you provide is encrypted Rev Environ Health. Therefore, a rapid and … Ondek Pty Ltd. and Helicobacter pylori Research Laboratory, Marshall Centre for Infectious Disease Research and Training, School of Pathology & Laboratory Medicine, Discipline of Microbiology & Immunology, The University of Western Australia, M504, L Block, QEII Medical Centre, Nedlands, WA, 6009 Australia PubMed Central Therefore, using a similar approach as in antibiotic resistance, this study investigates the potential use of the genomic approach to evaluate biofilm formation. Understanding the mechanism of biofilm formation is essential for improving H. pylori elimination strategies. The significant variant that we found were adjacent to the variable region of alpB that previously reported to play role in the biofilm formation (locus 121–150) [38]. Roary: rapid large-scale prokaryote pan genome analysis. Price MN, Dehal PS, Arkin AP. Overall, the rapid test showed a poor sensitivity (63.8%-71.1%), but a good specificity (91.1%-96.2%) before treatment. 2022 May 2;13:858247. doi: 10.3389/fmicb.2022.858247. Structure and function of the archaeal response regulator CheY. These genes regulate the change of helical form to coccoid forms which are commonly observed in biofilm of H. pylori [34, 35]. Epub 2017 May 5. 2015;31(22):3691–3. Google Scholar. 2013;30(6):1454–64. sharing sensitive information, make sure you’re on a federal J Clin Med. Genome Biol. 2018;110(1):114–27. The sensitivity and specificity of the HPS tests, rapid urease test, 13C-UBTs, and histology were 71.43% and 55.42%, 76.19% and 98.80%, 100% and 92.77%, and 85.71% and 95.18%, respectively, relative to the gold standard. Yoshio Yamaoka. Konstantopoulos N, Rüssmann H, Tasch C, Sauerwald T, Demmelmair H, Autenrieth I, Koletzko S. Am J Gastroenterol. Reilly TG, Poxon V, Sanders DS, Elliott TS, Walt RP. 2018;115(6):E1259-E68. Biofilm formation is a beneficial mechanism that requires complex regulation and interaction between bacteria. Utility of Esophagogastroduodenoscopy in the Evaluation of Uncomplicated Abdominal Pain in Children. Article The genes were present if the percent coverage was more than 50% and the percent identity was more than 90%. Infect Immun. MwSt. Google Scholar. Review of current diagnostic methods and advances in. The dots show the P-value of each variant, and 18 dots above the line have a P-value less than 0.05. The analysis discovered that 28% of the DUF1524 was absent in the high biofilm former [25]. A genome-wide Helicobacter pylori morphology screen uncovers a membrane-spanning helical cell shape complex. The measurement of the well-containing medium devoid of bacteria was used as a negative control. Argimón S, Abudahab K, Goater RJ, Fedosejev A, Bhai J, Glasner C et al. The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates. Your US state privacy rights, This result was supported by other study reported that there is no association between specific lineages or population with biofilm formation [28]. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. Quax TEF, Altegoer F, Rossi F, Li Z, Rodriguez-Franco M, Kraus F et al. Proc Natl Acad Sci U S A. 2009;24(1):1–14. Gut. Bogiel T, Mikucka A, Szaflarska-PopÅawska A, Grzanka D. Int J Mol Sci. PLoS ONE. Meanwhile, the lowest number of variant were present in cheY gene that regulates the direction of flagella motor Due to its critical role in survival, motility related gene has been conserved among archae and bacterial [31]. 2018;13(8):e0200064. 2016. Multiple H. pylori infections … The isolates used as the primary dataset in this study were obtained from gastric biopsies of patients from a survey involving 133 subjects from Dhaka Medical College Hospital in 2014 [46]. The morphology of biofilm formation on the coverslip surface on the day 4 of culture can be seen in this figure (10,000X magnification). These cookies will be stored in your browser only with your consent. 1999;28(2):121–6. Results: The straight horizontal line represents the cut-off for the significant P-value (0.05). Am J Gastroenterol. Methods: 2002;51(6):495–502. 2016;1(5):1–2. 2021 Nov 7;27(41):7100-7112. doi: 10.3748/wjg.v27.i41.7100. Nevertheless, further study should be done to identify polymorphism of the targeted genes that may affect the phenotype. 2005 Jun;43(6):2598-601. doi: 10.1128/JCM.43.6.2598-2601.2005. Microtiter plate assay methods of classification of bacterial biofilm formation. This similar approach is potentially applied to the other phenotype, such as the bacteria’s ability to form biofilm. Clipboard, Search History, and several other advanced features are temporarily unavailable. Several putative adhesion molecules, including BabA, SabA, Lewis×antigen, and other outer membrane proteins (OMPs) are ready to adhere … The gene expression of the alpB gene was also higher in the high biofilm former compared to low biofilm former isolates (Supplementary Figure S3). The first step of the analysis only targetting the well-known mutation. The site is secure. The other non-parametric correlation was analyzed using Spearman’s rank correlation model. The updated JSPGHAN guidelines for the management of Helicobacter pylori infection in childhood. Among the low-biofilm former, 11 strains of the low-biofilm formers had OD below control. Jones JM, Grinberg I, Eldar A, Grossman AD. 2022 Dec 22;24(1):179. doi: 10.3390/ijms24010179. We conclude that the new office based monoclonal enzyme immunoassay for diagnosis of H. pylori should be modified to improve sensitivity, inter-observer-variability and some technical problems. 2013;62(3):394–9. Pediatr Int. Tuan VP, Narith D, Tshibangu-Kabamba E, Dung HDQ, Viet PT, Sokomoth S et al. Google Scholar.